Teaching Electrophysiology & Imaging for Graduate Students
Neuronal Signals (NBDS 5123, previously NBDS 5161)
Course Director & Instructor:
Abdallah Hayar
One
Credit–Hour, Fall 2016, Time: 3:20 pm-4:50 pm
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|
Session |
Day |
Date |
Location |
Topic |
Instructor |
|
1 |
Mon |
4/18/2016 |
Biomed II, 6th floor, Conf. Rm |
Design of an electrophysiology setup |
Hayar |
|
2 |
Tue |
4/19/2016 |
Biomed II, 6th floor, Conf. Rm |
Neural population recordings |
Hayar |
|
3 |
Thu |
4/21/2016 |
Biomed I, 5th floor, Conf. Rm |
Single cell recordings |
Hayar |
|
4 |
Tue |
4/26/2016 |
Biomed I, Rm 205/207 |
Analyzing synaptic activity |
Hayar |
|
5 |
Wed |
4/27/2016 |
Biomed I, Rm 205/207 |
Data acquisition and analysis |
Hayar |
|
6 |
Fri |
4/29/2016 |
Biomed I, Rm 205/207 |
Analyzing and plotting data using OriginLab |
Hayar |
|
7 |
Tue |
5/3/2016 |
Biomed II, 6th floor, Conf. Rm |
Detecting electrophysiological events |
Hayar |
|
8 |
Thu |
5/5/2016 |
Biomed II, 6th floor, Conf. Rm |
Writing algorithms in OriginLab |
Hayar |
|
9 |
Fri |
5/6/2016 |
Biomed II, 6th floor, Conf. Rm |
Imaging neuronal activity |
Hayar |
|
10
|
Tue
|
5/10/2016
|
Biomed II, 6th floor, Conf. Rm |
-Exam &
Students’ Survey
of the course
-Laboratory
demonstration of an electrophysiology and imaging experiment |
Hayar
|
Course Description:
This
condensed one credit summer course consists of teaching the theoretical aspects
of modern analytical methods to study neuronal activity. It focuses on the study
of brain signals and describes several methods for recording and analyzing
neuronal activity using modern techniques such as patch clamping and imaging
neuronal networks using calcium- and voltage-sensitive dyes. The course is to be
taught annually in June-July, during a 7–week period on Tuesdays and Thursdays.
It consists of 12 sessions: 9 lectures (80 min each), 1 laboratory demonstration
(80 min) and 2 article presentations (80 min each) by the instructor. The goal
of the course is to help graduate and medical students pursuing a career in
neuroscience or neurology to become familiar with the cutting edge techniques
for monitoring brain activity.
Course Objectives:
·
To recognize and know
the functions of major electrophysiological recording devices.
·
To be able to
describe the significance and organization of an electrophysiological
laboratory.
·
To know how to design
electrophysiology setups and experimental protocols to investigate brain
activity.
·
To be able to
articulate the basic theoretical underpinnings of techniques currently used for
monitoring brain activity.
·
To enhance the
ability to critically analyze journal articles related to the fields of
electrophysiology and imaging.
Useful Documents:
Axon_Guide-version3.pdf
Axopatch200B_manual_Rev_D.pdf
Chp04-Optical Recording from Individual
Neurons in Culture.PDF
Chp16-Optical Recording from Populations
of Neurons in Brain Slices.PDF
Digidata1322A_manual_Rev_D.pdf
Digidata_1440A_Manual_Rev_A.pdf
HandbookofOpticalFilters.pdf
MultiClamp_700B_Manual_RevD.pdf
pCLAMP9.0_manual_Rev_C.pdf
pCLAMP10-User-Guide-RevA.pdf
MiniAnalysis603.EXE
One
Credit–Hour, Fall 2013, Time: 11:30 am-1:00 pm
Location:
Biomedical Research Building II, 6th floor, conference room,
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One
Credit–Hour, Summer 2010, Time: 9:00
-10:20 am
NBDS Sudents - Class 2010
15 students were enrolled, 7 of them were auditors
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