Teaching Electrophysiology & Imaging for Graduate Students
Neuronal Signals (NBDS 5123, previously NBDS 5161)

 Course Director & Instructor: Abdallah Hayar


One Credit–Hour, Fall 2016, Time: 3:20 pm-4:50 pm


Click on Topic to download lectures

Session

Day

Date

Location

Topic

Instructor

1

Mon

4/18/2016

Biomed II, 6th floor, Conf. Rm

Design of an electrophysiology setup

Hayar

2

Tue

4/19/2016

Biomed II, 6th floor, Conf. Rm

Neural population recordings

Hayar

3

Thu

4/21/2016

Biomed I, 5th floor, Conf. Rm

Single cell recordings

Hayar

4

Tue

4/26/2016

Biomed I, Rm 205/207

Analyzing synaptic activity

Hayar

5

Wed

4/27/2016

Biomed I, Rm 205/207

Data acquisition and analysis

Hayar

6

Fri

4/29/2016

Biomed I, Rm 205/207

Analyzing and plotting data using OriginLab

Hayar

7

Tue

5/3/2016

Biomed II, 6th floor, Conf. Rm

Detecting electrophysiological events

Hayar

8

Thu

5/5/2016

Biomed II, 6th floor, Conf. Rm

Writing algorithms in OriginLab

Hayar

9

Fri

5/6/2016

Biomed II, 6th floor, Conf. Rm

Imaging neuronal activity

Hayar

10

 

Tue

 

5/10/2016

 

Biomed II, 6th floor, Conf. Rm

 

-Exam & Students’ Survey of the course
-Laboratory demonstration of an electrophysiology and imaging experiment

Hayar

 

Course Description: This condensed one credit summer course consists of teaching the theoretical aspects of modern analytical methods to study neuronal activity. It focuses on the study of brain signals and describes several methods for recording and analyzing neuronal activity using modern techniques such as patch clamping and imaging neuronal networks using calcium- and voltage-sensitive dyes. The course is to be taught annually in June-July, during a 7–week period on Tuesdays and Thursdays. It consists of 12 sessions: 9 lectures (80 min each), 1 laboratory demonstration (80 min) and 2 article presentations (80 min each) by the instructor. The goal of the course is to help graduate and medical students pursuing a career in neuroscience or neurology to become familiar with the cutting edge techniques for monitoring brain activity.

Course Objectives:

  To recognize and know the functions of major electrophysiological recording devices.

  To be able to describe the significance and organization of an electrophysiological laboratory.

  To know how to design electrophysiology setups and experimental protocols to investigate brain activity.

  To be able to articulate the basic theoretical underpinnings of techniques currently used for monitoring brain activity.

  To enhance the ability to critically analyze journal articles related to the fields of electrophysiology and imaging.

 

Useful Documents:

Axon_Guide-version3.pdf

Axopatch200B_manual_Rev_D.pdf

Chp04-Optical Recording from Individual Neurons in Culture.PDF

Chp16-Optical Recording from Populations of Neurons in Brain Slices.PDF

Digidata1322A_manual_Rev_D.pdf

Digidata_1440A_Manual_Rev_A.pdf

HandbookofOpticalFilters.pdf

MultiClamp_700B_Manual_RevD.pdf

pCLAMP9.0_manual_Rev_C.pdf

pCLAMP10-User-Guide-RevA.pdf

MiniAnalysis603.EXE


One Credit–Hour, Fall 2013, Time: 11:30 am-1:00 pm

Location: Biomedical Research Building II, 6th floor, conference room,


Click on Topic to download lectures

Session

Day

Date

Topic

Instructor

1

Tue

9/3/2013

Design of an electrophysiology setup

Hayar

2

Wed

9/4/2013

Neural population recordings

Hayar

3

Thu

9/5/2013

Single cell recordings

Hayar

4

Fri

9/6/2013

Analyzing synaptic activity

Hayar

5

Mon

9/9/2013

Data acquisition and analysis

Hayar

6

Tue

9/10/2013

Analyzing and plotting data using OriginLab

Hayar

7

Wed

9/11/2013

Detecting electrophysiological events

Hayar

8

Thu

9/12/2013

Writing algorithms in OriginLab

Hayar

9

Fri

9/13/2013

Imaging neuronal activity

Hayar

10

 

Mon

 

9/16/2013

 

-Exam and Students’ Survey of the course
-Laboratory demonstration of an electrophysiology and imaging experiment

Hayar

 

 


One Credit–Hour, Summer 2010, Time: 9:00 -10:20 am
NBDS Sudents - Class 2010

15 students were enrolled, 7 of them were auditors

 

Click on Topic to download lectures

Session

Day

Date

Topic

Instructor

1

Tue

6/1

Design of an electrophysiology setup

Hayar

2

Thu

6/3

Neural population recordings

Hayar

3

Thu

6/10

Single cell recordings

Hayar

4

Fri

6/11

Analyzing synaptic activity

Hayar

5

Mon

6/14

Data acquisition and analysis

Hayar

6

Wed

6/16

Analyzing and plotting data using OriginLab

Hayar

7

Fri

6/18

Detecting electrophysiological events

Hayar

8

Mon

6/21

Writing algorithms in OriginLab

Hayar

9

Wed

6/23

Imaging neuronal activity

Hayar

10

 

Fri

 

6/25

 

-Exam and Students’ Survey of the course
-Laboratory demonstration of an electrophysiology and imaging experiment

Hayar

 

 


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